GenixTalk

  From 850,000yen

Production service for monoclonal antibody using mice

<Service Features>

• Immunization using 5 BALB/c mice
• Various screening methods available : ELISA test, Western blotting, Immunoprecipitation
  (When choosing Western blotting or Immunoprecipitation, clients are required to prepare the
  test samples and the certain fees will be charged.)
• When requested, culture supernatant provided for test
• Minimum two positive clones provided according to the screening methods requested by the client.
• Ascites production available using hybridoma cells. (for an extra charge)
• Period : 18-26 weeks (included peptide synthesis)
• The period can be shorter based on the immunization methods. (Inquire for more information)

Process for monoclonal antibody

Step	Lead time	Order	Process	Remarks
I	8 weeks	1	Choose the animals for experiment.	Choose the mice.
		2	Immunity	Conduct 3 immune treatments to 5 mice for 4 weeks. (At 2 weeks from the second immune treatment, take the blood sample. The antibody according to ELISA is measured: Data are sent.) (Three days after third immune treatment, take out spleens) for 5 mice.
II	2 weeks	3	Prepare the cell fusion.	We are checking FCS.
		4	Cell fusion	Conduct the fusion of Myeloma and spleen by using the PEG solution (1 day)
		5	Make the HAT culture medium.	Choose the best Hybridoma from each type of Hybridoma.
		6	Screening according to ELISA.	Measure the antibodies of the selected Hybridoma according to ELISA 14 days after the cell fusion.
III	4 weeks	7	Cloning	After measuring the positive well with the primary screening, conduct the cloning by using the limiting culture.
		8	Establish Hybridoma	Sub cloning (primary, secondary) (Measure the antibodies according to ELISA: Data are sent.)
		9	Freeze and preserve	We send 2 frozen clones.

<Client Requirements>

• Protein 2.5 mg (purity : 90-95%) or peptide 5 mg (purity : about 70%)
• Antigen information (M.W., toxicity, buffer, concentration etc.)