Synthesis option & price
- Polyclonal antibody kit
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Polyclonal antibody kit used 2 rabbits ------- from 240,000 yen
- Free peptide prediction
- Peptide synthesis
- Immunity : 4times
- Peptide conjugation
- Immunization trial/final serum
Conduct 4 times of immunization used 2 rabbits, and measured ELISA data before primary
immunization, after 1st, 3rd boosting for checking the titer. Finally obtain serum. - Affinity purification (option)
- Free peptide prediction
- Peptide synthesis
- Immunity : 4times
- Peptide conjugation
- Immunization trial/final serum
Conduct 4 times of immunization used 5 mice, and measured ELISA data before primary
immunization, after 1st, 3rd boosting for checking the titer. Finally heart puncture will be performed. - Affinity purification (option)
- Monoclonal antibody
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Process for monoclonal antibody From 850,000yen
Step Lead time Order Process Remarks I 8 weeks 1 Choose the animals for experiment. Choose the mice. 2 Immunity Conduct 3 immune treatments to 5 mice for 4 weeks.
(At 2 weeks from the second immune treatment, take the blood sample. The antibody according to ELISA is measured: Data are sent.)
(Three days after third immune treatment, take out spleens) for 5 mice.II 2 weeks 3 Prepare the cell fusion. We are checking FCS. 4 Cell fusion Conduct the fusion of Myeloma and spleen by using the PEG solution (1 day) 5 Make the HAT culture medium. Choose the best Hybridoma from each type of Hybridoma. 6 Screening according to ELISA. Measure the antibodies of the selected Hybridoma according to ELISA 14 days after the cell fusion. III 4 weeks 7 Cloning After measuring the positive well with the primary screening, conduct the cloning by using the limiting culture. 8 Establish Hybridoma Sub cloning (primary, secondary)
(Measure the antibodies according to ELISA: Data are sent.)9 Freeze and preserve We send 2 frozen clones.
- Antibody purification service
- High-grade purification service using serum or ascites.
- Antigen specific purification
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Methods Column chromatography (2 step) Chromatography resin 1) Antigen specific affinity column 2) Protein A or G column Serum amount 10 ml Period 2-3 weeks - Total lgG purification
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Methods Column chromatography (1 step) Chromatography resin Protein A column (Rabbit lgG) or Protein G column (Mouse lgG) Serum amount 10 ml Period 2-3 weeks
- Serum or ascites amounts of 10ml that are being purified.
- Antigen (Protein 2.5mg, Peptide 5mg, only when chosen for antigen specific antibody)
Click on "antibody purification protocol" for more information.
| Product name | Price | Order |
|---|---|---|
| Polyclonal antibody kit used 2rabbits( included free peptide prediction) | 240,000yen | Order |
| Polyclonal antibody kit used 2rabbits( excluded free peptide prediction) | 240,000yen | Order |
| Polyclonal antibody kit used 5mice ( included free peptide prediction) | 210,000yen | Order |
| Polyclonal antibody kit used 5mice ( excluded free peptide prediction) | 210,000yen | Order |
| Monoclonal antibody | 850,000yen | Order |
| Affinity purification (option) | 850,000yen | Order |
| IgG purification (option) | 54,000yen | Order |
How to process after placing an order
1. Place an order using the cart.
2. We will communicate with you regarding the peptide sequence and process schedule through e-mail after within a few days.
3. When everything is confirmed, we will start the process.