GenixTalk

Antibodies are reagents used in ELISA, Western blotting, Immuprecipitation, Immunohistochemistry and Immunocytochemistry for having the property of antigen specific affinity. GenixTalk's antibody production service using 2 rabbits and 5 mice helps our clients with the antigen preparation and antibody purification that along with the production service can expand the purpose and use of antibodies for our clients.
*The GenixTalk serves by creating one integrated kit from the manufacturing of peptide antigen by searching epitopes to manufacturing polyclonal antibody.

Rabbit polyclonal antibody

Production service that provides polyclonal antibody using rabbits.
Click on "each service" for more information

Mouse polyclonal antibody kit

From 210,000yen

Polyclonal antibody production service using mice

<Service Features>

• The GenixTalk serves by creating one integrated kit from the synthesis of peptide antigen by searching epitopes to manufacturing polyclonal antibody.
• For peptide order16AA~20AA, additional 8500JPY is required.
• Immunity : 4 times
• ELISA test : attach titer data according to the ELISA test of every trial serum and final serum.
• Form of delivery: After serum, deliver the antiserum in a frozen form after serum.
• Delivery due date : before/after 16 weeks
• Confidentiality Agreement: When a customer requires, we sign a confidentiality agreement.
      Although it is required to respond flexibly at any condition, please consult us first.
• Remarks: Please give separate orders to respond flexibly to matters such as delivery time.

Polyclonal antibody schedule used 5 mice

Step	Lead time	Order	Process		Remarks
I	5weeks	1	Free peptide prediction		When peptide is used as antigen, we synthesize 12-15mer peptides in regard to antigenicity and solubility. If you give us the full sequence of the protein you wish to isolate for your antibody, we will send you the sequence prediction for free of charge. Period: 4days
		2	Peptide synthesis as an antigen &cojugation		10mg, 80%, 15AA KLH BSA conjugation (5mg) * Conjugation service to provide coupling of KLH (Keyhole Limpet Haemocyanin) as a carrier protein with peptide to create booster immunogens. Service Features For Immunization : KLH conjugation For ELISA test : BSA conjugation Period: 1 week
II	10weeks	3	Immunity (5 mice)	Primary injection	Used 0.1mg of Antigen peptide 4weeks
				1st boosting	Used 0.1mg of Antigen peptide 1st tiral serum by ELISA TEST
				2nd boosting	Used 0.1mg of Antigen peptide 2nd tiral serum by ELISA TEST
				3nd boosting	Used 0.1mg of Antigen peptide 3rd tiral serum by ELISA TEST
III	1week	4	Affinitiy purification(option)