CEM™ custom RNA: 50mer and longer custom RNA
GenixTalk provides high-quality long RNA oligomers synthesized by using the new CEM™-amidite method.
- GenixTalk
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The CEM™ method allows the synthesis of long RNA oligomers of more than 100 nucleotides.
Synthesis of a very long RNA oligomer (110mer) . -
The CEM™ method can give the desired RNA oligomers in high yield, with a 99% coupling efficiency at each step.
CEM™ method Conventional method Comparison of purity of crude RNA (55mer) on HPLC. -
In the CEM™ method, further purification of the crude reaction mixture gives long oligomers of very high purity.
HPLC (A) and electrophoresis (B) of purified 110mer RNA. [References]
1) T. Ohgi et al., Org. Lett., 2005, 7, 3477-3480.
2) Y. Shiba et al., Nucleic Acids Res., 2007, 35, 3287-3296.
3) Y. Shiba et al., Nucleic Acids Res. Symp. Ser., 2006, 50, 11-12.
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The CEM™ method allows the synthesis of long RNA oligomers of more than 100 nucleotides.
(Synthetic scale)
50 nmol, 200 nmol, 400 nmol, 1µmol (* See the table below for the calculated expected yield at different synthetic scales)
| Length of synthetic oligomer | 20mer | 50mer | 110mer |
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| Yield | 91% | 78% | 58% |
| 50 nmol | 46 nmol | 39 nmol | 29 nmol |
| 200 nmol | 182 nmol | 156 nmol | 116 nmol |
| 400 nmol | 364 nmol | 312 nmol | 232 nmol |
| 1000 nmol | 910 nmol | 780 nmol | 580 nmol |
| Supply of data | Upon your request, we can provide HPLC and mass spectrometric data at an additional charge. |
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| Delivery | The time required for delivery will depend on the sequence. We will let you know by e-mail. There will be a delivery charge of US$50 per order. |
Synthetic options:
Lineup of modifications available.
Address:
2-7, Tenjinbashi 1-chome, Kita-Ku, Osaka 530-0041, Japan
Tel: + 81-6-6352-5673 (for Japanese customers)
+ 82-2-583-4485 (for international customers)
Fax: + 81-6-6353-0075 (for Japanese customers)
+ 82-2-583-4487 (for international customers)
E-mail: order@genixtalk.com