Unique statistical sequential design
- Sequence design image by GenixTalk
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( Flow of design )
1) As for the target genes, the candidate sequence of 21 bases are done by alternating each other. 2) For each candidate sequence, the candidate sequence is selected by predicting the KD (knock down) active score from unique algorithm and considering the various parameters. 3) At last, the low ordered candidates are removed from blast searching..
* The prediction algorithm is our unique design method in consideration of the method of Teramoto (FEBS Letters 579(2005) 2878-2882) and operating the three kinds of learning methods and also considering the sequence of Tm value, GC%, G/C and A/U.
More accurate purity is predicted by increasing the learning data, according to the data feedback. - Specific inhibition effect of design sequence of GenixTalk
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(A)
We measure the inhibition effects on cell of RAW 264.7 with RT-PCR after 2 days after transfecting the siRNA which is designed on the target gene (TLR4) by GenixTalk. RAW264.7. The TLR4 mRNA volume is inhibited by 20% compared with the Mock cell (treated with the same method by removing siRNA0, ensuring that it inhibits the growth of protein at the same time.
(B)
If we compare the genes RAW264.7 cell and the cell gene of TLR4 Knockdown with the micro array having the 22,000 genes, we can find out that the TLR4 inside the target gene is inhibited by a half. At this time, the genes having changed two times or more were only 7 including TLR4. (If we compare the untreated cell with the mock cell with the same preparation, the number of genes having changing two times or more was 11. The siRNA sequence which is designed by GenixTalk inhibits the target genes that the global genes were not affected at all.